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KMID : 0545120030130060984
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Journal of Microbiology and Biotechnology
2003 Volume.13 No. 6 p.984 ~ p.988
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Purification and Identification of an Antifungal Agent from Streptomyces sp. KH-614 Antagonistic to Rice Blast Fungus, Pyricularia oryzae
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RHEE KI-HYEONG
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Abstract
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The actinomycete strain KH-6 14 possessed strong antifungal activity, especially antagonistic to the rice blast fungus, Pyricularia orvzae. Diaminopimelic acid (DAP) type and morphological and physiological characteristics, examined by scanning electron microscopy (SEM). indicated that KH- 6 14 belonged to the genus Streptornyc´es. Antifungal agent produced by this strain was found to be most active. when the strain was cultured in the presence of glucose, polypeptone, and yeast extract (PY) medium for 6 days at 27℃. Rased on the spectral report data, MS and NMR, the antifungal agent was identified as cyclo(L-leucyl-L-prolyl). According to the antimicrobial activity test measured by minimal inhibitory concentration (MIC), the cyclo(leu-pro) exhibited the activity against Cutzdidu dbiciins 1AM 4905. Mucor rmzunnianus IAM62 18, Rhizoctoriia solani IF0 62 18. Asper~ilii.s,fiimigntu.s ATCC 42202. Glnrnerella citz~ulatn IF0 9767. Trichophtori mentagrophytes ATCC 1 8749. and Trichophytorz nihri´im ATCC 44766. the order of MIC values were 50, 12.5, 5. 50, 25. 5, 5 kg/ml, respectively. Specifically, cyclo(leu-pro) was one of the most effective elements against Pyriculariti oryzae IF0 5994 with the MIC value of 2.5 pg/ml, thus indicating that cyclo(1eu-pro) is a potential antifungal agent.
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KEYWORD
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